rat il-1β elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: ZH reduces IMQ-induced inflammatory responses and abnormal proliferation in mice. A Representative spleen images and spleen weight quantification. B Immunohistochemical (IHC) staining of Ki67 in skin lesions (left panel) and quantification of its AOD (right panel). C qRT-PCR analysis of Il-22 , Il-1β , Tnf-α , and Ccl20 mRNA expression in lesion skin tissues. D ELISA quantification of IL-22, IL-1β, TNF-α, and CCL20 protein levels in lesion skin tissues. n = 6 (biological replicates). Data are analyzed using one-way ANOVA followed Tukey’s post hoc test. ** P < 0.01 vs. Control group; # P < 0.05; ## P < 0.01 vs. IMQ group

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Immunohistochemical staining, Immunohistochemistry, Quantitative RT-PCR, Analysis, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: ZH alters gut microbiota composition and function in IMQ-induced mice. A Random forest analysis ranking bacterial taxa based on their importance in group classification using the Gini index. B Relative abundances of the top-ranked taxa across different groups. C Pearson correlation analysis between significantly altered bacterial taxa and skin inflammatory cytokines, including IL-22, IL-1β, TNF-α, and CCL20. * P < 0.05, ** P < 0.01

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Analysis

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: Gut microbiota depletion attenuates the therapeutic effects of ZH against IMQ-induced psoriasis-like inflammation. A Schematic diagram of the experimental design for antibiotic-mediated gut microbiota depletion and subsequent establishment of the IMQ-induced psoriasis-like dermatitis model. B Quantification of fecal bacterial abundance by qPCR to assess the efficacy of antibiotic-mediated gut microbiota depletion. C Representative images of dorsal skin lesions in mice from the indicated groups. D PASI scores for scaling, erythema, thickness, and total scores. E Representative images of spleens (left) and quantification of spleen weight (right). F Representative images of H&E-stained dorsal skin sections (left) and quantification of epidermal thickness (right). G Representative immunohistochemical staining of Ki67 in dorsal skin lesions (left) and quantification of Ki67-positive cells (right). H Relative mRNA expression levels of Il-22 , Il-1β , Tnf-α , and Ccl20 in dorsal skin lesions determined by qPCR. I Protein levels of IL-22, TNF-α, IL-1β, and CCL20 in dorsal skin lesions determined by ELISA. For panels B, the Control and ABX groups each contained 12 mice before randomization; for panels C-I, mice were randomly allocated into four groups ( n = 6 per group): IMQ, IMQ + ABX, IMQ + M-ZH, and IMQ + M-ZH + ABX. Data are analyzed using one-way ANOVA followed Tukey’s post hoc test. * P < 0.05, ** P < 0.01 vs. IMQ group; # P < 0.05, ## P < 0.01 vs. IMQ + ABX group; & P < 0.05, && P < 0.01 vs. IMQ + M-ZH group

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Staining, Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Cardiovascular Therapeutics

Article Title: The miR‐511‐3p/ATP2A2 Axis Is Required for Propofol‐Mediated Cardioprotection Against Hypoxia/Reoxygenation Injury

doi: 10.1155/cdr/7021184

Figure Lengend Snippet: Effects of propofol intervention on oxidative stress and inflammatory factors in H/R‐stimulated cardiomyocytes. (A) Intracellular malondialdehyde (MDA) levels in H9c2 cells of each group determined by colorimetric kits. (B) ELISA detection of superoxide dismutase (SOD) expression in cells of each group. (C) Relative reactive oxygen species (ROS) contents in cells of each group measured using fluorescent probes. (D) ELISA quantification of tumor necrosis factor‐ α (TNF‐ α ) concentrations in cell culture supernatant of each group. (E) ELISA detection of interleukin‐6 (IL‐6) concentrations in cell culture supernatant of each group. (F) ELISA measurement of interleukin‐1 β (IL‐1 β ) concentrations in cell culture supernatant of each group. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: ELISA kits for rat creatine kinase‐MB (CK‐MB), heart‐type fatty acid‐binding protein (H‐FABP), tumor necrosis factor‐ α (TNF‐ α ), interleukin‐6 (IL‐6), and interleukin‐1 β (IL‐1 β ) were purchased from Elabscience (China), and the rat superoxide dismutase (SOD) kit was sourced from Jianglai Biotechnology (China).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture